Product name: Human Coagulation Factor VIII
Application:ELISA, Inhibition Assays, Western Blotting
Presentation:100μl purified human factor VIII protein at 100μg/ml in 50mM Tris-Acetate, pH7.5, 1mM EDTA, 20% Glycerol without BSA and Sodium Azide
Description:
The human coagulation factor VIII is assayed by its biological activity as a cofactor in the activation of factor X by activated factor IX (factor IXa) in the presence of calcium ions and phospholipids. The assay consists of two consecutive steps: the factor VIII-dependent activation of factor X and the enzymatic cleavage of a chromogenic factor Xa substrate that can be quantified photometrically. Many commercial kits are available for this purpose
Factor VIII (FVIII) is an essential clotting factor in the coagulation cascade. The lack of normal FVIII causes Hemophilia A, an inherited bleeding disorder. FVIII harvested from donated blood/blood plasma or recombinant FVIII can be given to hemophiliacs to restore hemostasis. Thus, FVIII is also known as Anti-Hemophilic Factor
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Human Coagulation Factor VIII
